Interaction of bromocresol green with different serum albumins studied by fluorescence quenching
نویسندگان
چکیده
منابع مشابه
Interaction of bilirubin with lipids studied by fluorescence quenching method.
The interaction of bilirubin and various lipids was studied by a static fluorescence quenching method. A quenching equation was developed to determine the binding constant. This method was tested first by a 5-dimethylaminoaphthalene-1-sulfonyl (dansyl).bovine albumin system and then applied to a fluorescent probe containing lipid. Both systems can yield binding parameters. For certain lipid sys...
متن کاملMeasurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods.
BACKGROUND/AIMS The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), ...
متن کاملLaboratory Exercises Protein Tryptophan Accessibility Studied by Fluorescence Quenching
A laboratory class is described to introduce the biochemistry major students to the basic concepts and various applications of fluorescence spectroscopy. Through simple and inexpensive experiments the students learn how to record excitation and emission spectra and measure intrinsic protein fluorescence and its quenching to elucidate the local tryptophan environment. Free tryptophan, ovalbumin,...
متن کاملFluorescence Quenching of Green Fluorescent Protein during Denaturation by Guanidine
Fluorescence of green fluorescent protein mutant, 2-5 GFP is observed during denaturation by guanidine. The fluorescence intensity decreases exponentially but the fluorescence lifetime does not change during denaturation. The fluorescence lifetime of the denatured protein is shorter than that of native form. As the protein structure is modified by guanidine, solvent water molecules penetrate in...
متن کاملComparative studies on the interaction of cefixime with bovine serum albumin by fluorescence quenching spectroscopy and synchronous fluorescence spectroscopy.
Under simulated physiological conditions, the reaction mechanism between cefixime and bovine serum albumin at different temperatures (293, 303 and 310 K) was investigated using a fluorescence quenching method and synchronous fluorescence method, respectively. The results indicated that the fluorescence intensity and synchronous fluorescence intensity of bovine serum albumin decreased regularly ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: IUBMB Life
سال: 1997
ISSN: 1521-6543
DOI: 10.1080/15216549700203751